Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Ratchathewi, Bangkok, 10400, Thailand.
Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
BMC Oral Health. 2024 Feb 12;24(1):223. doi: 10.1186/s12903-024-03978-4.
Secreted protein acidic and rich in cysteine (SPARC) has been shown to modulate aggressive behavior in several benign and malignant tumors. Little is known about SPARC expression in odontogenic keratocyst (OKC), an odontogenic cyst with an aggressive nature. To the best of our knowledge, only one study has been investigated the expression of this protein in OKCs. This study aimed to characterize SPARC expression in OKCs. Additionally, to determine whether SPARC is associated with aggressive behavior in OKCs, SPARC expression in OKCs was compared with radicular cysts (RCs), dentigerous cysts (DCs) and calcifying odontogenic cysts (COCs). These odontogenic cysts showed no or less aggressive behavior.
SPARC expression was evaluated in 38 OKCs, 39 RCs, 35 DCs and 14 COCs using immunohistochemistry. The percentages of positive cells and the intensities of immunostaining in the epithelial lining and the cystic wall were evaluated and scored.
Generally, OKCs showed similar staining patterns to RCs, DCs and COCs. In the epithelial lining, SPARC was not detected, except for ghost cells in all COCs. In the cystic wall, the majority of positive cells were fibroblasts. Compared between 4 groups of odontogenic cysts, SPARC expression in OKCs was significantly higher than those of RCs (P < 0.001), DCs (P < 0.001) and COCs (P = 0.001).
A significant increase of SPARC expression in OKCs compared with RCs, DCs and COCs suggests that SPARC may play a role in the aggressive behavior of OKCs.
富含半胱氨酸的酸性分泌蛋白（SPARC）已被证明可调节多种良性和恶性肿瘤的侵袭性行为。关于牙源性角化囊肿（OKC）中 SPARC 的表达知之甚少，OKC 是一种具有侵袭性的牙源性囊肿。据我们所知，仅有一项研究调查了这种蛋白质在 OKC 中的表达。本研究旨在研究 OKC 中 SPARC 的表达特征。此外，为了确定 SPARC 是否与 OKC 的侵袭性行为相关，将 OKC 中的 SPARC 表达与根侧囊肿（RCs）、含牙囊肿（DCs）和牙源性钙化囊肿（COCs）进行了比较。这些牙源性囊肿表现出无侵袭性行为或侵袭性行为较弱。
采用免疫组织化学法检测 38 例 OKC、39 例 RCs、35 例 DCs 和 14 例 COCs 中 SPARC 的表达。评估并评分上皮衬里和囊壁中阳性细胞的百分比和免疫染色强度。
OKC 的染色模式通常与 RCs、DCs 和 COCs 相似。在上皮衬里中，除了所有 COCs 的鬼影细胞外，均未检测到 SPARC。在囊壁中，大多数阳性细胞为成纤维细胞。在 4 组牙源性囊肿之间进行比较，OKC 中的 SPARC 表达明显高于 RCs（P<0.001）、DCs（P<0.001）和 COCs（P=0.001）。
与 RCs、DCs 和 COCs 相比，OKC 中 SPARC 的表达显著增加，这表明 SPARC 可能在 OKC 的侵袭性行为中发挥作用。
