[中国四川一个家族的家族性系统性红斑狼疮患者蛋白质组学研究]

[Study on proteomics of familial systemic lupus erythematosus patients in one family from Sichuan, China].

作者信息

Wu Yong-kang, Huang Zhuo-chun, Shi Yun-ying, Cai Bei, Wang Lan-lan, Ying Bin-wu, Hu Chao-jun, Li Yong-zhe

机构信息

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 May;40(3):504-7, 512.

PMID:19627015

Abstract

OBJECTIVE

To investigate the proteomic characteristics of systemic lupus erythematosus (SLE) in a SLE family from Sichuan, China which consisting of 7 members with 3 SLE cases, and to find the proteins correlated with the heredity of SLE.

METHODS

A total of 153 serum samples were collected from 7 members including 3 SLE sisters in this SLE family, 63 individual SLE patients, as well as 83 healthy controls. The diagnosis of SLE is based on the American College of Rheumatology criteria (1997). All serum samples were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with magnetic beads technology. Serum protein profiles were obtained by MALDI-TOF-MS combined with magnetic beads in order to identify predictive biomarkers of risk of suffering SLE. The resulting spectra were analyzed with Biomarker Wizard software 3.1.0.

RESULTS

Four discriminative mass/charge (m/z) proteins serving as pathogenic biomarkers were identified on arrays for family SLE cases versus individual SLE and healthy controls. The protein level of peak intensities at m/z of 9342.23 was significantly greater in SLE family group compared with that in individual SLE patients and healthy controls (P<0.05), those of individual SLE patients were significantly greater compared with healthy controls (P<0.05); the proteins level of peak intensities at m/z of 4094.03, 5905.35 and 7973.53 in SLE family group were significantly lower compared with that in individual SLE patients and healthy controls (P<0.05), those of individual SLE patients were significantly lower compared with healthy controls (P<0.05).

CONCLUSION

The proteins of m/z of 9342.23, 4094.03, 5905.35 and 7973.53 maybe play a great role in assemble pathogenesis of SLE and predict the risk of suffering SLE. The higher protein level of m/z of 9342.23 and the lower protein level of m/z of 4094.03, 5905.35 and 7973.53, the higher risk of sufferring with SLE.

摘要

目的

研究来自中国四川一个系统性红斑狼疮（SLE）家族（共7名成员，其中3例SLE患者）的SLE蛋白质组学特征，寻找与SLE遗传相关的蛋白质。

方法

从该SLE家族的7名成员（包括3名SLE姐妹）、63例SLE个体患者以及83名健康对照中总共采集了153份血清样本。SLE的诊断基于美国风湿病学会标准（1997年）。所有血清样本采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）结合磁珠技术进行分析。通过MALDI-TOF-MS结合磁珠获得血清蛋白质谱，以鉴定SLE患病风险的预测生物标志物。所得光谱用Biomarker Wizard软件3.1.0进行分析。

结果

在家族性SLE病例与个体SLE及健康对照的阵列上，鉴定出4种作为致病生物标志物的差异质荷比（m/z）蛋白质。与个体SLE患者和健康对照相比，SLE家族组中m/z为9342.23处的峰强度蛋白质水平显著更高（P<0.05），个体SLE患者的该蛋白质水平显著高于健康对照（P<0.05）；SLE家族组中m/z为4094.03、5905.35和7973.53处的峰强度蛋白质水平显著低于个体SLE患者和健康对照（P<0.05），个体SLE患者的该蛋白质水平显著低于健康对照（P<0.05）。