Towns J K, Regnier F E
Department of Chemistry, Purdue University, West Lafayette, IN 47907.
J Chromatogr. 1990 Sep 7;516(1):69-78. doi: 10.1016/s0021-9673(01)90205-5.
A hydrophilic, positively charged, durable coating has been developed for capillary electrophoresis of macromolecules. Polyethyleneimine is adsorbed to the inner wall of fused silica capillaries and the adsorbed coating cross-linked into a stable layer. Capillaries of polyethyleneimine-coated silica gave unique separations owing to the reversal of electro-osmotic flow caused by the positively charged coating. The resulting coating was stable from pH 2-12 and could be used over a wide pH range without substantial change in electro-osmotic flow. High-molecular-weight polymers were needed to give thick coatings which mask silanol groups on the wall. Proteins were resolved quickly and efficiently with good recovery using capillaries of 50 cm in length.
一种用于大分子毛细管电泳的亲水性、带正电荷的耐用涂层已被开发出来。聚乙烯亚胺吸附在熔融石英毛细管的内壁上，吸附的涂层交联形成稳定层。由于带正电荷的涂层导致电渗流反转，涂有聚乙烯亚胺的硅胶毛细管实现了独特的分离。所得涂层在pH 2至12范围内稳定，可在较宽的pH范围内使用，而电渗流没有实质性变化。需要高分子量聚合物来形成厚涂层，以掩盖壁上的硅醇基团。使用50厘米长的毛细管，蛋白质能够快速、高效地分离，回收率良好。
