Sánchez-Guadarrama Obdulia, López-Sandoval Horacio, Sánchez-Bartéz Francisco, Gracia-Mora Isabel, Höpfl Herbert, Barba-Behrens Noráh
Departamento de Química Inorgánica, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, D.F. México, Mexico.
J Inorg Biochem. 2009 Sep;103(9):1204-13. doi: 10.1016/j.jinorgbio.2009.05.018. Epub 2009 Jun 16.
Herein we present the synthesis, structural and spectroscopic characterization of coordination compounds of cobalt(II), copper(II) and zinc(II) with 2-methylbenzimidazole (2mbz), 2-phenylbenzimidazole (2phbz), 2-chlorobenzimidazole (2cbz), 2-benzimidazolecarbamate (2cmbz) and 2-guanidinobenzimidazole (2gbz). Their cytotoxic activity was evaluated using human cancer cell lines, PC3 (prostate), MCF-7 (breast), HCT-15 (colon), HeLa (cervic-uterine), SKLU-1 (lung) and U373 (glioblastoma), showing that the zinc(II) and copper(II) compounds [Zn(2mbz)(2)Cl(2)].0.5H(2)O, [Zn(2cmbz)(2)Cl(2)].EtOH, [Cu(2cmbz)Br(2)].0.7H(2)O and [Cu(2gbz)Br(2)] had significant cytotoxic activity. The isostructural cobalt(II) complexes showed not significant activity. The cytotoxic activity is related to the presence of halides in the coordination sphere of the metal ion. Recuperation experiments with HeLa cells, showed that the cells recuperated after removing the copper(II) compounds and, on the contrary, the cells treated with the zinc(II) compounds did not. These results indicate that the mode of action of the coordination compounds is different.
在此，我们展示了钴（II）、铜（II）和锌（II）与2-甲基苯并咪唑（2mbz）、2-苯基苯并咪唑（2phbz）、2-氯苯并咪唑（2cbz）、2-苯并咪唑氨基甲酸酯（2cmbz）和2-胍基苯并咪唑（2gbz）形成的配位化合物的合成、结构和光谱表征。使用人癌细胞系PC3（前列腺）、MCF-7（乳腺）、HCT-15（结肠）、HeLa（宫颈-子宫）、SKLU-1（肺）和U373（胶质母细胞瘤）评估了它们的细胞毒性活性，结果表明锌（II）和铜（II）化合物[Zn(2mbz)(2)Cl(2)].0.5H(2)O、[Zn(2cmbz)(2)Cl(2)].EtOH、[Cu(2cmbz)Br(2)].0.7H(2)O和[Cu(2gbz)Br(2)]具有显著的细胞毒性活性。同构的钴（II）配合物显示出不显著的活性。细胞毒性活性与金属离子配位球中卤化物的存在有关。对HeLa细胞的恢复实验表明，去除铜（II）化合物后细胞恢复，相反，用锌（II）化合物处理的细胞则没有。这些结果表明配位化合物的作用方式不同。
