Chen Bor-Yann, Yen Chia-Yi, Chen Wen-Ming, Chang Chan-Tang, Wang Chin-Tsan, Hu Yuh-Chung
Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 260, Taiwan.
Bioresour Technol. 2009 Dec;100(23):5763-70. doi: 10.1016/j.biortech.2009.06.080. Epub 2009 Jul 22.
This follow-up study provided an evaluation on threshold operation criteria of biostimulation in immobilized cell systems (ICSs) with Aeromonas hydrophila onto packing materials Porites corals. Essential nutrients in appropriate flow rate for biostimulation were inevitably required to maintain maximum attached cell population for cost-effective biodecolorization. With the method of "graphical reconstruction", the most economically feasible strategy of medium stimulation for color removal was quantitatively revealed. Our findings pointed out no matter what operation mode of reactor was (e.g., suspended batch cultures or ICS) color removal efficiency for A. hydrophila still strongly depended upon intrinsic kinetics and chemical reactivities of azo dyes. Mass transport effects in ICS might not play most significant roles to limit dye biodecolorization of A. hydrophila (except Reactive red 198, Reactive green 19), as relative rankings of color removal rates of various dyes were almost in parallel with those in suspended batch cultures.
这项后续研究对嗜水气单胞菌固定化细胞系统（ICSs）在多孔珊瑚填料上进行生物刺激的阈值操作标准进行了评估。为了实现具有成本效益的生物脱色，维持最大附着细胞数量不可避免地需要以适当流速提供基本营养物质用于生物刺激。通过“图形重建”方法，定量揭示了用于脱色的最经济可行的培养基刺激策略。我们的研究结果指出，无论反应器的操作模式如何（例如，悬浮分批培养或ICS），嗜水气单胞菌的脱色效率仍然强烈依赖于偶氮染料的内在动力学和化学反应性。ICS中的传质效应可能并非限制嗜水气单胞菌染料生物脱色的最重要因素（活性红198、活性绿19除外），因为各种染料脱色率的相对排名几乎与悬浮分批培养中的排名平行。
