College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, People's Republic of China.
Luminescence. 2010 Jan-Feb;25(1):50-4. doi: 10.1002/bio.1143.
The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of glutamine (Gln) using a flow-injection (FI) system. Gln was found to strongly inhibit the CL signal of the luminol-H(2)O(2)-CuSO(4) system in Na(2)B(4)O(7) solution. A new FI-CL method was developed for the determination of Gln. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimized conditions, the corresponding linear regression equation was established over the range of 5.0 x 10(-7) to 2.5 x 10(-6) mol/L with the detection limit of 1.8 x 10(-8) mol/L. The relative standard deviation was found to be 1.8% for 11 replicate determinations of 1.5 x 10(-6) mol/L Gln. The proposed method has been satisfactorily applied for the determination of Gln in real samples (Marzulene-s granules) with recoveries in the range of 98.7-108.6%. The minimum sampling rate was about 100 samples/h. The possible mechanism of this inhibitory CL was studied by fluorescence spectrophotometer and UV-vis spectrophotometer.
本研究的主要目的是开发一种使用流动注射（FI）系统的廉价、简单、快速和灵敏的化学发光（CL）方法来测定谷氨酰胺（Gln）。在硼酸钠溶液中，Gln 被发现强烈抑制鲁米诺-H2O2-CuSO4 体系的 CL 信号。建立了一种新的 FI-CL 方法来测定 Gln。系统地优化了影响重现性和 CL 检测的参数。在优化条件下，建立了 5.0×10（-7）至 2.5×10（-6）mol/L 范围内的相应线性回归方程，检测限为 1.8×10（-8）mol/L。1.5×10（-6）mol/L Gln 的 11 次重复测定的相对标准偏差为 1.8%。该方法已成功应用于实际样品（Marzulene-s 颗粒）中 Gln 的测定，回收率在 98.7-108.6%之间。最小采样率约为 100 个样品/小时。通过荧光分光光度计和紫外可见分光光度计研究了这种抑制性 CL 的可能机制。
