Sergeev M N, Spirichev V B
Biokhimiia. 1990 Aug;55(8):1527-31.
In vitamin B6-deficient rats the concentration of in vivo occupied nuclear and total cellular receptors of 1.25(OH)2D3 increases 1.3-1.7 times, whereas the binding of in vitro occupied receptors to DNA-cellulose increases by 40%. Pyridoxal-5'-phosphate (PLP) added in vitro to solubilized receptors of 1.25(OH)2D3 lowers the ligand binding by 15-25% but causes no dissociation of hormone-receptor complexes formed in vivo. The association of in vitro occupied receptors of 1.25(OH)2D3 with DNA-cellulose is suppressed by PLP (3.5-4.5-fold). It has been shown for the first time that vitamin B6 is a physiological regulator of 1.25(OH)2D3 receptor binding by chromatin and DNA which diminish the concentration of occupied receptors and thus suppress the hormonal response.
在维生素B6缺乏的大鼠中，1,25-二羟维生素D3（1,25(OH)2D3）体内占据的核受体和总细胞受体浓度增加1.3至1.7倍，而体外占据的受体与DNA纤维素的结合增加40%。体外添加到1,25(OH)2D3溶解受体中的磷酸吡哆醛（PLP）使配体结合降低15%至25%，但不会导致体内形成的激素-受体复合物解离。PLP抑制体外占据的1,25(OH)2D3受体与DNA纤维素的结合（3.5至4.5倍）。首次表明维生素B6是1,25(OH)2D3受体与染色质和DNA结合的生理调节剂，可降低占据受体的浓度，从而抑制激素反应。
