Mody I, Reynolds J N, Salter M W, Carlen P L, MacDonald J F
Department of Neurology and Neurological Sciences M016, Stanford University School of Medicine, CA 94305.
Brain Res. 1990 Oct 29;531(1-2):88-94. doi: 10.1016/0006-8993(90)90761-y.
Single electrode voltage-clamp recordings were obtained from dentate gyrus granule cells (GCs) in hippocampal slices of control and commissurally kindled rats. Two types of calcium currents, a transient and a sustained current, were studied in control and kindled neurons. The threshold of the transient calcium current was lowered in kindled GCs. The sustained calcium current was absent in kindled neurons but it could be restored by the intracellular administration of the calcium chelator EGTA. Our findings are consistent with the hypothesis that the loss of an intraneuronal calcium binding protein (Calbindin-D28K; CaBP) reduces the intraneuronal calcium buffering capacity in kindled neurons and results in the enhanced calcium-dependent inactivation of sustained calcium currents.
从对照大鼠和经连合部点燃大鼠的海马切片齿状回颗粒细胞（GCs）中获得单电极电压钳记录。在对照神经元和点燃神经元中研究了两种类型的钙电流，即瞬态电流和持续电流。在点燃的GCs中，瞬态钙电流的阈值降低。点燃的神经元中不存在持续钙电流，但通过细胞内给予钙螯合剂EGTA可以恢复。我们的发现与以下假设一致：神经元内钙结合蛋白（钙结合蛋白-D28K；CaBP）的缺失降低了点燃神经元中神经元内钙缓冲能力，并导致持续钙电流的钙依赖性失活增强。
