限制聚合酶链反应假阳性结果：检测DNA和mRNA以区分活菌与死菌。
Limiting false-positive polymerase chain reaction results: detection of DNA and mRNA to differentiate viable from dead bacteria.
作者信息
Kobayashi Hideo, Oethinger Margret, Tuohy Marion J, Procop Gary W, Hall Gerri S, Bauer Thomas W
机构信息
Pathology and Laboratory Medicine Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
出版信息
Diagn Microbiol Infect Dis. 2009 Aug;64(4):445-7. doi: 10.1016/j.diagmicrobio.2009.04.004.
We compared 2 methods for determining Escherichia coli viability in vitro. A 16S rDNA polymerase chain reaction (PCR) assay detected bacteria irrespective of viability. A groEL mRNA reverse transcriptase PCR was positive for 72 h but later became negative. Detecting mRNA holds promise but is tedious, and groEL may not be the best target.
我们比较了两种体外测定大肠杆菌活力的方法。一种16S rDNA聚合酶链反应（PCR）检测法能检测出细菌，无论其是否具有活力。一种groEL mRNA逆转录PCR在72小时内呈阳性，但之后变为阴性。检测mRNA有前景，但操作繁琐，而且groEL可能不是最佳靶点。
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