Beck R, Rawet M, Wieland F T, Cassel D
Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520, USA.
FEBS Lett. 2009 Sep 3;583(17):2701-9. doi: 10.1016/j.febslet.2009.07.032. Epub 2009 Jul 22.
Transport of membranes and proteins in eukaryotic cells is mediated by vesicular carriers. Here we review the biogenesis and functions of COPI vesicles, carriers that operate in the early secretory pathway. We focus on mechanisms mediating coat recruitment, uptake of cargo, vesicle budding and fission, and finally dissociation of the coat. In this context, recent findings on the interplay between machinery and auxiliary proteins in COPI vesicle formation and function will be discussed. Specifically, we will weigh the pros and cons of recent data on roles of the small GTP binding protein Arf1, of Arf1GAPs, and lipids during COPI carrier formation.
真核细胞中膜和蛋白质的运输由囊泡载体介导。在此，我们综述COP I囊泡的生物发生及其功能，COP I囊泡是在早期分泌途径中起作用的载体。我们重点关注介导衣被募集、货物摄取、囊泡出芽和裂变，以及最终衣被解离的机制。在此背景下，将讨论关于COP I囊泡形成和功能中机制蛋白与辅助蛋白之间相互作用的最新发现。具体而言，我们将权衡关于小GTP结合蛋白Arf1、Arf1GAPs和脂质在COP I载体形成过程中作用的最新数据的利弊。
