Xu J L, Khor K A, Sui J J, Zhang J H, Chen W N
School of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
Biomaterials. 2009 Oct;30(29):5385-91. doi: 10.1016/j.biomaterials.2009.07.002. Epub 2009 Jul 23.
The use of synthetic hydroxyapatite as bone substitute calls for the knowledge of the influence on adjacent cells. The aim of this study was to investigate the proteins with differential protein expression levels in the proteome of human osteoblast cell line incubated separately with various nano sized hydroxyapatite powders with different shapes and chemical compositions using iTRAQ-coupled 2D LC-MS/MS approach. In the present study, we investigated several intracellular signaling molecules involved in calcium regulation to analyze how osteoblast cells respond to dissimilar HA nanoparticles. It was found there was a significant decrease in cell population after adding the HA nanoparticles to the osteoblasts. Our results combining proteomics analysis and RT-PCR validation on targeted genes involved in calcium regulation confirmed the differences in the cellular response to dissimilar HA nanoparticles.
使用合成羟基磷灰石作为骨替代物需要了解其对相邻细胞的影响。本研究的目的是使用iTRAQ耦合二维液相色谱-串联质谱法，研究人成骨细胞系蛋白质组中与不同形状和化学成分的各种纳米级羟基磷灰石粉末分别孵育时蛋白质表达水平存在差异的蛋白质。在本研究中，我们研究了几种参与钙调节的细胞内信号分子，以分析成骨细胞如何对不同的羟基磷灰石纳米颗粒作出反应。结果发现，向成骨细胞中添加羟基磷灰石纳米颗粒后，细胞数量显著减少。我们结合蛋白质组学分析和对参与钙调节的靶向基因进行逆转录-聚合酶链反应验证的结果，证实了细胞对不同羟基磷灰石纳米颗粒的反应存在差异。
