具有反向重复序列的钠离子同向转运体的结构与功能
Structure and function of Na(+)-symporters with inverted repeats.
作者信息
Abramson Jeff, Wright Ernest M
机构信息
Department of Physiology, David School of Medicine at UCLA, Los Angeles, CA 90095-1751, USA.
出版信息
Curr Opin Struct Biol. 2009 Aug;19(4):425-32. doi: 10.1016/j.sbi.2009.06.002. Epub 2009 Jul 22.
Abstract
Symporters are membrane proteins that couple energy stored in electrochemical potential gradients to drive the cotransport of molecules and ions into cells. Traditionally, proteins are classified into gene families based on sequence homology and functional properties, for example the sodium glucose (SLC5 or Sodium Solute Symporter Family, SSS or SSF) and GABA (SLC6 or Neurotransmitter Sodium Symporter Family, NSS or SNF) symporter families [1-4]. Recently, it has been established that four Na(+)-symporter proteins with unrelated sequences have a common structural core containing an inverted repeat of 5 transmembrane (TM) helices [5()-8()]. Analysis of these four structures reveals that they reside in different conformations along the transport cycle providing atomic insight into the mechanism of sodium solute cotransport.
摘要
同向转运体是一种膜蛋白，它利用电化学势梯度中储存的能量来驱动分子和离子协同转运进入细胞。传统上，蛋白质是根据序列同源性和功能特性被分类到基因家族中的，例如钠葡萄糖（SLC5或钠溶质同向转运体家族，SSS或SSF）和GABA（SLC6或神经递质钠同向转运体家族，NSS或SNF）同向转运体家族[1-4]。最近，已经确定四种序列不相关的Na(+)同向转运体蛋白具有一个共同的结构核心，该核心包含一个由5个跨膜（TM）螺旋组成的反向重复序列[5()-8()]。对这四种结构的分析表明，它们在转运循环中处于不同的构象，从而为钠溶质协同转运机制提供了原子层面的见解。
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