Wulf-Johansson Helle, Hay-Schmidt Anders, Poulsen Asser Nyander, Klaerke Dan Arne, Olesen Jes, Jansen-Olesen Inger
Department of Neurology and Danish Headache Center, Glostrup Research Institute, Glostrup Hospital, Faculty of Health Sciences, University of Copenhagen, 2600 Glostrup, Denmark.
Brain Res. 2009 Oct 6;1292:1-13. doi: 10.1016/j.brainres.2009.07.047. Epub 2009 Jul 23.
Large conductance calcium-activated potassium (BK(Ca)) channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. Migraine has been proposed to be a trigeminovascular disease involving the sensory trigeminal pathways and the cerebral arteries. We hypothesize that BK(Ca) channel alpha- and beta-subunits are present in the rat and porcine trigeminal ganglion (TG) thus enabling a role in migraine. BK(Ca) channel mRNA was detected using reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization. BK(Ca) channel protein was visualized by western blotting and histochemistry. The presence of the modulatory beta1-beta 4 subunit mRNAs was investigated using RT-PCR. beta1-, beta2- and beta 4-subunit mRNAs were expressed in rat TG whereas beta2- and beta 4-subunits were detected in porcine TG. Western blotting revealed beta2- and beta 4-subunit proteins in rat and porcine TG. The present study showed BK(Ca) channel expression in rat and porcine TG. The main modulatory beta-subunits detected in TG of both species were beta2- and beta 4-subunits.
大电导钙激活钾（BK(Ca)）通道有助于电冲动、信息的正常信号传递以及神经递质释放的调节。偏头痛被认为是一种涉及三叉神经感觉通路和脑动脉的三叉神经血管性疾病。我们假设BK(Ca)通道的α亚基和β亚基存在于大鼠和猪的三叉神经节（TG）中，因此在偏头痛中发挥作用。使用逆转录聚合酶链反应（RT-PCR）和原位杂交检测BK(Ca)通道mRNA。通过蛋白质印迹法和组织化学观察BK(Ca)通道蛋白。使用RT-PCR研究调节性β1-β4亚基mRNA的存在情况。β1、β2和β4亚基mRNA在大鼠TG中表达，而β2和β4亚基在猪TG中被检测到。蛋白质印迹法显示大鼠和猪TG中存在β2和β4亚基蛋白。本研究表明BK(Ca)通道在大鼠和猪TG中表达。在两个物种的TG中检测到的主要调节性β亚基是β2和β4亚基。
