Xi Liu, Junjian Zhang, Yumin Liu, Yunwen Luo, Hongbin Wen
Department of Neurology, Zhangnan Hospital, Wuhan University, Donghu Road 169#, Wuhan 430071, China.
Neurosci Lett. 2009 Sep 29;463(1):6-11. doi: 10.1016/j.neulet.2009.07.056. Epub 2009 Jul 23.
To investigate the biomarkers of vascular cognitive impairment (VCI), we analyzed 30 VCI patients and 30 healthy controls using proteome technology. Peptides in sera were purified using magnetic beads, and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and ClinProTool software. Twenty-four peptides were significantly differently expressed between VCI patients and controls. There were seven peptides selected for our diagnostic model. The sensitivity of the model measured by internal and external validation was 95% and 80% respectively, while the specificity was 100% for both. A peptide peak at 4963 m/z was chosen for identification by nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC-ESI-MS/MS). The amino acid sequence was consistent with the fragment of the trace-amine associated receptor 6 (TAAR6) in the human international protein index database. In conclusion, the specific fragment of TAAR6 may be important for clinical biomarkers in the diagnosis of VCI. The high specificity and sensitivity of the model may provide an appropriate method for screening VCI patients.
为了研究血管性认知障碍（VCI）的生物标志物，我们使用蛋白质组技术分析了30例VCI患者和30名健康对照者。血清中的肽段用磁珠进行纯化，并用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和ClinProTool软件进行分析。VCI患者和对照者之间有24种肽段表达存在显著差异。我们的诊断模型选取了7种肽段。通过内部和外部验证测得的模型灵敏度分别为95%和80%，而特异性均为100%。选择4963 m/z处的一个肽峰通过纳升液相色谱-电喷雾电离-串联质谱（nano-LC-ESI-MS/MS）进行鉴定。其氨基酸序列与人类国际蛋白质索引数据库中痕量胺相关受体6（TAAR6）的片段一致。总之，TAAR6的特定片段可能对VCI诊断中的临床生物标志物很重要。该模型的高特异性和灵敏度可能为筛选VCI患者提供一种合适的方法。
