Dailey Tanya, Ji Huiling, Long Vit, Chien Edward K
Section of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Warren Alpert Medical School of Brown University, Providence, RI, USA.
Am J Obstet Gynecol. 2009 Sep;201(3):322.e1-6. doi: 10.1016/j.ajog.2009.06.002. Epub 2009 Jul 24.
Transforming growth factor beta (TGFbeta) plays a central role in extracellular matrix remodeling. We hypothesized that TGFbeta signaling is involved in cervical remodeling. This study evaluated patterns within this signaling pathway.
The cervices of nonpregnant and timed pregnant rats were obtained. Messenger ribonucleic acid (mRNA) expression of TGFbeta1, TGFbeta receptor 1 (TbetaR1), TbetaR2, and TbetaR3 was evaluated. Four animals were euthanized for each time point. Western blotting was performed for protein expression. Phosphorylated mothers against decapentaplegic (Smad)-2 and -3 phosphorylation was assessed to evaluate TGFbeta activation.
TGFbeta1 mRNA increased through day 21 and declined on day 22 (analysis of variance, P = .001). TbetaR1 expression was unchanged. TbetaR2 and TbetaR3 mRNA expression was similar to TGFbeta1. TbetaR3 protein expression was similar to mRNA. Smad2 phosphorylation paralleled changes in TbetaR3.
Components of the TGFbeta signaling pathway increase during pregnancy along with Smad2 activation. The decline on day 22 correlates with a transition to the ripening phase supporting a role in cervical remodeling.
转化生长因子β（TGFβ）在细胞外基质重塑中起核心作用。我们假设TGFβ信号传导参与宫颈重塑。本研究评估了该信号通路中的模式。
获取未孕和定时妊娠大鼠的子宫颈。评估TGFβ1、TGFβ受体1（TβR1）、TβR2和TβR3的信使核糖核酸（mRNA）表达。每个时间点处死4只动物。进行蛋白质印迹法检测蛋白质表达。评估磷酸化的母亲对脱磷酸化蛋白（Smad）-2和-3的磷酸化以评估TGFβ激活情况。
TGFβ1 mRNA在第21天前增加，在第22天下降（方差分析，P = .001）。TβR1表达未改变。TβR2和TβR3 mRNA表达与TGFβ1相似。TβR3蛋白质表达与mRNA相似。Smad2磷酸化与TβR3的变化平行。
TGFβ信号通路的成分在妊娠期间随着Smad2激活而增加。第22天的下降与向成熟阶段的转变相关，支持其在宫颈重塑中的作用。
