Department of Applied Chemistry, National University of Kaohsiung, No. 700, Kaohsiung University Road, Nan Tzu District, Kaohsiung 811, Taiwan.
J Chromatogr A. 2009 Dec 25;1216(52):9034-47. doi: 10.1016/j.chroma.2009.07.026. Epub 2009 Jul 18.
Nanometer-sized gold particles-gold nanoparticles (Au NPs)-are attracting a great deal of attention for their use in various technologies, including catalysis, optical and electronic devices, and separation science. In the emerging field of nanomaterials, the design, synthesis, and characterization of nanostructures are critical features because the manipulation of these structures has a direct effect on their resulting macroscopic properties. Nanostructures fabricated in layers on surfaces-for example, through self-assembly processes-have several potential applications in separation science. This review provides an introduction to the characterizations of Au NPs using size exclusion chromatography, high performance liquid chromatography (HPLC), and electrophoresis, and their self-assembly onto solid supports for analyses based on HPLC, gas chromatography, and capillary electrophoresis. In addition, sample concentration strategies involving the use of self-assembly approaches for surface modification of Au NPs are also discussed.
纳米级金颗粒-金纳米粒子（Au NPs）-因其在各种技术中的应用而引起了广泛关注，包括催化、光学和电子设备以及分离科学。在新兴的纳米材料领域中，对纳米结构的设计、合成和表征是关键特征，因为这些结构的操纵直接影响它们的宏观性质。通过自组装过程在表面上制造的分层纳米结构例如，在分离科学中有几种潜在的应用。本综述介绍了使用尺寸排阻色谱法、高效液相色谱法（HPLC）和电泳法对 Au NPs 的特性进行分析，以及它们在固体载体上的自组装用于基于 HPLC、气相色谱法和毛细管电泳的分析。此外，还讨论了涉及使用自组装方法进行 Au NPs 表面修饰的样品浓缩策略。
