Costantine Maged M, Ferrari Francesca, Chiossi Giusseppe, Tamayo Esther, Hankins Gary D V, Saade George R, Longo Monica
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX, USA.
Am J Obstet Gynecol. 2009 Sep;201(3):301.e1-6. doi: 10.1016/j.ajog.2009.05.040. Epub 2009 Jul 26.
To determine whether the intrauterine environment affects the postnatal vascular response to stress in a model of fetal programming induced by endothelial nitric oxide synthase deficiency.
Homozygous nitric oxide synthase knockout and wild-type controls were crossbred to obtain maternally and paternally derived heterozygous offspring. At 14 weeks of age, in vivo blood pressure measurements by telemetry, and in vitro carotid arteries vascular reactivity studies were performed in male offspring after subjecting them to shaker stress.
Maternally derived heterozygous offspring, compared with paternally derived heterozygous offspring, had significantly higher systolic blood pressure, mean arterial blood pressure, and pulse pressure before, as well as after introduction of the shaker stress. The difference in the latter between maternally and paternally derived heterozygous offspring was accentuated after stress. Maternally derived heterozygous offspring also had significantly higher contractile responses to phenylephrine when compared with paternally derived heterozygous offspring, and this was abolished after incubation with L-NAME.
The adverse uterine environment affects the postnatal vascular response to stress.
在内皮型一氧化氮合酶缺乏诱导的胎儿编程模型中，确定子宫内环境是否会影响出生后血管对应激的反应。
将纯合子一氧化氮合酶基因敲除小鼠与野生型对照进行杂交，以获得来自母本和父本的杂合子后代。在14周龄时，对雄性后代施加振动应激后，通过遥测技术进行体内血压测量，并进行体外颈动脉血管反应性研究。
与来自父本的杂合子后代相比，来自母本的杂合子后代在引入振动应激之前和之后，收缩压、平均动脉压和脉压均显著更高。应激后，来自母本和父本的杂合子后代在这方面的差异更加明显。与来自父本的杂合子后代相比，来自母本的杂合子后代对去氧肾上腺素的收缩反应也显著更高，而在用L-精氨酸甲酯孵育后这种反应消失。
不良的子宫环境会影响出生后血管对应激的反应。
