Schneider Christian, Jira Thomas
Institute of Pharmacy, Pharmaceutical/Medicinal Chemistry, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Str. 17, D-17487 Greifswald, Germany.
J Chromatogr A. 2009 Aug 28;1216(35):6285-94. doi: 10.1016/j.chroma.2009.07.004. Epub 2009 Jul 9.
Calixarene-bonded stationary phases in HPLC are known to support additional interactions compared to conventional alkyl-bonded phases (pi-pi interactions, complex-building interactions). Thus it cannot be presumed that the same mechanisms of retention apply and that retention can be predicted in similar ways. Here 31 solutes of highly various molecular structures have been analysed at different mobile phase compositions (0-98% (v/v) methanol) in order to characterise the chromatographic behaviour of the novel stationary phases and to test the applicability of established models predicting retention factors. The influence of a change of the methanol content is discussed for non-polar, polar and ionic solutes and differences of their behaviour on the differing column types are shown. Additionally estimates about underlying retention mechanisms are given.
已知在高效液相色谱中，杯芳烃键合固定相相比于传统的烷基键合相能支持额外的相互作用（π-π相互作用、络合物形成相互作用）。因此，不能假定相同的保留机制适用，也不能假定可以以类似的方式预测保留情况。在这里，为了表征新型固定相的色谱行为并测试预测保留因子的既定模型的适用性，已在不同的流动相组成（0-98%（v/v）甲醇）下分析了31种分子结构差异很大的溶质。讨论了甲醇含量变化对非极性、极性和离子型溶质的影响，并展示了它们在不同柱型上行为的差异。此外，还给出了对潜在保留机制的估计。
