Kurihara Yukio, Kaminuma Eli, Matsui Akihiro, Kawashima Makiko, Tanaka Maho, Morosawa Taeko, Ishida Junko, Mochizuki Yoshiki, Shinozaki Kazuo, Toyoda Tetsuro, Seki Motoaki
Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan.
Plant Cell Physiol. 2009 Sep;50(9):1715-20. doi: 10.1093/pcp/pcp109. Epub 2009 Jul 24.
MicroRNAs (miRNAs) are 20-24 nucleotide endogenous regulatory molecules conserved in higher eukaryotes. In Arabidopsis, miRNAs are produced through step-wise cleavages of primary miRNA precursors (pri-miRNAs) by DICER-LIKE1 (DCL1). This cleavage step is also supported by a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1). In many cases, mature miRNA is predominantly incorporated into an endonuclease, ARGONAUTE1 (AGO1), which degrades miRNA-targeted mRNAs. Here, we examined and revealed whole genome transcriptomes in ago1-25 and hyl1-2 mutants using tiling arrays. The data in this paper are valuable for understanding the relationship between the miRNA pathway and its effect on transcriptomes.
微小RNA（miRNA）是在高等真核生物中保守的20 - 24个核苷酸的内源性调节分子。在拟南芥中，miRNA是通过DICER-LIKE1（DCL1）对初级miRNA前体（pri-miRNA）进行逐步切割产生的。这种切割步骤也受到双链RNA结合蛋白HYPONASTIC LEAVES1（HYL1）的支持。在许多情况下，成熟的miRNA主要被整合到核酸内切酶ARGONAUTE1（AGO1）中，AGO1会降解miRNA靶向的mRNA。在这里，我们使用平铺阵列检查并揭示了ago1-25和hyl1-2突变体中的全基因组转录组。本文中的数据对于理解miRNA途径及其对转录组的影响之间的关系具有重要价值。
