Division of Animal Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
Reproduction. 2009 Nov;138(5):771-81. doi: 10.1530/REP-08-0391. Epub 2009 Jul 24.
Different fatty acid (FA) sources are known to influence reproductive hormones in cattle, yet there is little information on how dietary FAs affect oocyte quality. Effects of three dietary sources of FAs (supplying predominantly palmitic and oleic, linoleic (n-6) or linolenic (n-3) acids) on developmental potential of oocytes were studied in lactating dairy cows. A total of 12 Holstein cows received three diets containing rumen inert fat (RIF), soyabean or linseed as the main FA source for three periods of 25 days in a Latin-square design. Within each period, oocytes were collected in four ovum pick-up sessions at 3-4 day intervals. FA profiles in plasma and milk reflected profiles of dietary FA sources, but major FAs in granulosa cells were not affected. Dietary FA source did not affect plasma concentrations of leptin, insulin, IGF1, GH, or amino acids. RIF led to a higher proportion of cleaved embryos than soya or linseed, but blastocyst yield and embryo quality were not affected. It is concluded that the ovary buffers oocytes against the effects of fluctuations in plasma n-3 and n-6 FAs, resulting in only modest effects on their developmental potential.
不同的脂肪酸（FA）来源已知会影响牛的生殖激素，但关于膳食 FA 如何影响卵母细胞质量的信息却很少。本研究探讨了三种膳食 FA 来源（主要提供棕榈酸和油酸、亚油酸（n-6）或亚麻酸（n-3））对泌乳奶牛卵母细胞发育潜力的影响。12 头荷斯坦奶牛接受了三种日粮，其中含有瘤胃惰性脂肪（RIF）、大豆或亚麻籽作为主要 FA 来源，每个周期持续 25 天，采用拉丁方设计。在每个周期内，每隔 3-4 天通过 4 次卵母细胞采集进行卵母细胞收集。血浆和乳中的 FA 图谱反映了膳食 FA 来源的图谱，但颗粒细胞中的主要 FA 不受影响。膳食 FA 来源不影响血浆中瘦素、胰岛素、IGF1、GH 或氨基酸的浓度。RIF 导致的分裂胚胎比例高于大豆或亚麻籽，但囊胚产量和胚胎质量不受影响。结论是，卵巢缓冲卵母细胞免受血浆中 n-3 和 n-6 FA 波动的影响，因此对其发育潜力的影响较小。
