Baranowska Irena, Kowalski Bartosz
Department of Analytical and General Chemistry, Chemical Faculty, Silesian University of Technology, 7 Marcina Strzody Street, 44-100 Gliwice, Poland.
Water Sci Technol. 2009;60(2):449-58. doi: 10.2166/wst.2009.339.
An analytical procedure for the determination of 10 drugs belonging to three different therapeutic groups was analysed. Several compounds were investigated which comprise of beta-blockers: sotalol (SOT), metoprolol (MET), propranolol (PRO), carvedilol (CAR); corticosteroids: prednisolone (PRE), dexamethasone (DEX); analgesic, non-steroidal anti-inflammatory (NSAID): paracetamol (PAR), metamizole (MTZ), aspirin (ASP) and ketoprofen (KET). Reversed-phase liquid chromatography with gradient elution, fluorescence (FL) and diode array detectors (DAD) was applied for the selective investigation of the drugs. Three solvents were used as the mobile phase: methanol, 0.05% trifluoroacetic acid (TFA) in water and acetonitrile. The solid phase extraction (SPE) method using the Oasis HLB column was proposed for the preconcentration step. The recovery level for most of the drugs was above 80%. The low values of limits of detection (LOD) for PAR, SOT, MTZ, MET, SAL, PRO, PRE, CAR, DEX and KET were achieved: 106 ng L(-1), 1,048 ng L(-1), 2.48 ng L(-1), 2.17 ng L(-1), 16.5 ng L(-1), 1.10 ng L(-1), 160 ng L(-1), 6.62 ng L(-1), 76.5 ng L(-1) and 45.2 ng L(-1), respectively. The suggested method was used for the determination of drugs in spiked surface water.
分析了一种用于测定属于三个不同治疗组的10种药物的分析方法。研究了几种化合物，其中包括β受体阻滞剂：索他洛尔（SOT）、美托洛尔（MET）、普萘洛尔（PRO）、卡维地洛（CAR）；皮质类固醇：泼尼松龙（PRE）、地塞米松（DEX）；镇痛、非甾体抗炎药（NSAID）：对乙酰氨基酚（PAR）、安乃近（MTZ）、阿司匹林（ASP）和酮洛芬（KET）。采用梯度洗脱的反相液相色谱法，结合荧光（FL）和二极管阵列检测器（DAD）对这些药物进行选择性研究。使用三种溶剂作为流动相：甲醇、0.05%的三氟乙酸（TFA）水溶液和乙腈。提出采用Oasis HLB柱的固相萃取（SPE）方法进行预浓缩步骤。大多数药物的回收率高于80%。实现了对PAR、SOT、MTZ、MET、SAL、PRO、PRE、CAR、DEX和KET的低检测限（LOD）值：分别为106 ng L⁻¹、1048 ng L⁻¹、2.48 ng L⁻¹、2.17 ng L⁻¹、16.5 ng L⁻¹、1.10 ng L⁻¹、160 ng L⁻¹、6.62 ng L⁻¹、76.5 ng L⁻¹和45.2 ng L⁻¹。所建议的方法用于测定加标地表水样品中的药物。
