Evans Cory J, Olson John M, Ngo Kathy T, Kim Eunha, Lee Noemi E, Kuoy Edward, Patananan Alexander N, Sitz Daniel, Tran Phuongthao, Do Minh-Tu, Yackle Kevin, Cespedes Albert, Hartenstein Volker, Call Gerald B, Banerjee Utpal
Department of Molecular, Cell and Developmental Biology, Los Angeles, University of California, Los Angeles, Los Angeles, California, USA.
Nat Methods. 2009 Aug;6(8):603-5. doi: 10.1038/nmeth.1356. Epub 2009 Jul 26.
We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
我们将Gal4-UAS、FLP重组酶-FRT和荧光报告基因相结合，以生成细胞克隆，这些克隆可提供有关黑腹果蝇中单个细胞起源的空间、时间和遗传信息。我们将这种组合命名为实时克隆表达的Gal4技术（G-TRACE）。该方法应允许在基因组规模上筛选和鉴定实时及谱系追踪的表达模式。
