蛋白激酶 C 抑制成年大鼠小直径背根神经节神经元 P2X 受体介导的内向电流。
Inhibition of P2X receptor-mediated inward current by protein kinase C in small-diameter dorsal root ganglion neurons of adult rats.
机构信息
Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
出版信息
Neurosci Bull. 2009 Aug;25(4):179-86. doi: 10.1007/s12264-009-0611-2.
OBJECTIVE
The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist beta, gamma-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons.
METHODS
Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats.
RESULTS
beta, gamma-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12, 13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner.
CONCLUSION
Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.
目的
本研究旨在验证三磷酸腺苷（ATP）对成年大鼠背根神经节（DRG）神经元的诱导的多种反应，并研究特定 P2X 受体激动剂 β,γ-亚甲基 ATP（β,γ-me-ATP）和蛋白激酶 C（PKC）对 DRG 神经元中 P2X 受体介导的内向电流的调制作用。
方法
采用全细胞膜片钳技术记录急性分离的成年大鼠 DRG 神经元的电流。
结果
β,γ-me-ATP 与 ATP 相似，以剂量依赖的方式诱发出 DRG 神经元中 2 种不同类型的 P2X 受体介导的内向电流。佛波醇 12,13-二丁酸酯（PDBu）激活 PKC 可显著抑制 P2X 受体介导的内向电流的 2 种亚型，呈剂量依赖性。
结论
PKC 的激活负调制大鼠 DRG 神经元中 P2X 受体介导的电流，这可能有助于预防在炎症或神经病变条件下伤害感受器的过度兴奋。
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