Institute of Biology, University of Iceland, Sturlugata, Reykjavík, Iceland.
Biochem Genet. 2009 Dec;47(11-12):817-30. doi: 10.1007/s10528-009-9280-0.
Developmental globin gene expression and gene switching in vertebrates have been extensively studied. Globin gene regions have been characterized in some fish species and show linked α and β loci. Understanding coordinated expression between α and β globin genes in fish is of importance for further insights into globin gene regulation in teleosts and higher vertebrates. We characterize linked β and α globin genes in Atlantic cod, pulled from the Atlantic cod genome with a PCR research strategy, by screening a genomic λ library and primer walking. The genes are oriented tail-to-head (5'-3'), differing from the head-to-head orientation in transcriptional polarity characteristic of teleostean globin genes. Four tandem repeats are found in an intergenic region of 1500 base pairs. One microsatellite, which consists primarily of atg tandem repeats, has an open reading frame. The globin genes and open reading frame have a CCAAT promoter element and TATA boxes. The promoters of the open reading frame and the β gene share an 89-bp block (with 100% identity) that probably regulates transcription.
脊椎动物的发育珠蛋白基因表达和基因转换已经得到了广泛的研究。一些鱼类物种的珠蛋白基因区域已经得到了描述，显示出连锁的α和β基因座。了解鱼类中α和β珠蛋白基因的协调表达对于进一步了解硬骨鱼类和高等脊椎动物的珠蛋白基因调控具有重要意义。我们通过筛选基因组 λ 文库和引物行走，使用 PCR 研究策略，从大西洋鳕鱼基因组中提取了连锁的β和α珠蛋白基因。这些基因的取向是尾到头（5'-3'），与转录极性特征的硬骨鱼珠蛋白基因的头到头取向不同。在 1500 个碱基对的基因间区发现了四个串联重复。一个微卫星主要由 atg 串联重复组成，具有开放阅读框。珠蛋白基因和开放阅读框具有 CCAAT 启动子元件和 TATA 盒。开放阅读框和β基因的启动子共享一个 89 个碱基对的块（具有 100%的同一性），可能调节转录。
