Smirnikhina S A, Lavrov A V
Mol Biol (Mosk). 2009 May-Jun;43(3):387-91.
The article provides analysis of common methods for DNA methylation detection. Advantages and limitations of methods used for different purposes are compared. Clue step for most common methods is bisulfite treatment of DNA samples and its protocol is described in details. Recommendations are formulated for each method best in solving specific problems.
本文对DNA甲基化检测的常用方法进行了分析。比较了用于不同目的的方法的优缺点。大多数常用方法的关键步骤是对DNA样本进行亚硫酸氢盐处理，并详细描述了其操作流程。针对每种最适合解决特定问题的方法提出了建议。
