Sun Hurong, Wei Lin, Liu Xiaoheng, Zeng Ye, Lai Yi, Yin Hongmei
Institute of Biomedical Engineering, West China College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Jun;26(3):512-7.
CXCR1 and CXCR2 are important receptors in regulating vascular endothelial cell activities. In order to elucidate the role of CXCR1/2 in shear stress-induced endothelial cell migration, we have investigated the expression levels of CXCR1 and CXCR2 in the endothelial cells exposed to shear stress. In the experiment, anti-IL8RA and anti-IL8RB were used to antagonize CXCR1 and CXCR2. Different shear stresses were generated in a flow chamber; scratch test was carried out to compare endothelial cell migration in the control group and the receptor-antagonized groups. The results indicated that the migration of endothelial cells was restrained effectively after CXCR1 and CXCR2 were antagonized by anti-IL8RA and anti-IL8RB. And anti-IL8RA showed a stronger inhibitive effect than did anti-IL8RB (P<0.05). In the group with both receptor antagonisms, the migration was further inhibited. These results suggest that both CXCR1 and CXCR2 are important factors in mediating the migration of endothelial cells induced by shear stress, and CXCR1 fulfills a more important role.
CXCR1和CXCR2是调节血管内皮细胞活性的重要受体。为了阐明CXCR1/2在剪切应力诱导的内皮细胞迁移中的作用，我们研究了暴露于剪切应力的内皮细胞中CXCR1和CXCR2的表达水平。在实验中，使用抗IL8RA和抗IL8RB拮抗CXCR1和CXCR2。在流动腔室中产生不同的剪切应力；进行划痕试验以比较对照组和受体拮抗组中内皮细胞的迁移。结果表明，用抗IL8RA和抗IL8RB拮抗CXCR1和CXCR2后，内皮细胞的迁移受到有效抑制。并且抗IL8RA显示出比抗IL8RB更强的抑制作用（P<0.05）。在两种受体均被拮抗的组中，迁移进一步受到抑制。这些结果表明，CXCR1和CXCR2都是介导剪切应力诱导的内皮细胞迁移的重要因素，并且CXCR1发挥更重要的作用。
