Wang Ying, Gao Zhenyue, Hu Yueyuan, Hu Qinghua, Yang Chun, Zhuang Fengyuan
Department of Bioengineering, Beihang University, Beijing 100083, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Jun;26(3):590-4, 605.
CHO cells expressing human GPIb/IX and rabbit red blood cells coated with human von Willebrand factor (VWF) were adapted to our study on the binding probability and the detachment force of GPIb/IX and VWF. With the micropipette system, the two cells were impinged under a constant force for controlled time. When the cells were pulled apart, the deformation of RBC was recorded, and the binding score and detachment force of the proteins were determined. After the two cells were impinged into 0.5 microm for 30 s, the binding probability of the two cells carrying GPIb/IX and VWF was 15.0%. Via analyzing the deformation of red blood cells, we found out the distribution of rupture forces of cells with GPIb/IX and VWF. Therefore, we infer that the continuous distribution of the detachment force is due to the stochastic effect. The most probable value of the detachment force was 10 pN.
表达人糖蛋白Ib/IX的中国仓鼠卵巢细胞（CHO细胞）和包被有人血管性血友病因子（VWF）的兔红细胞被用于我们关于糖蛋白Ib/IX与VWF的结合概率和分离力的研究。使用微量移液器系统，在恒定力作用下将这两种细胞撞击一定的时间。当细胞被拉开时，记录红细胞的变形情况，并测定蛋白质的结合分数和分离力。在将这两种细胞撞击0.5微米持续30秒后，携带糖蛋白Ib/IX和VWF的两种细胞的结合概率为15.0%。通过分析红细胞的变形情况，我们得出了带有糖蛋白Ib/IX和VWF的细胞破裂力的分布。因此，我们推断分离力的连续分布是由于随机效应。分离力的最可能值为10皮牛。
