Divrikli Umit, Akdogan Abdullah, Soylak Mustafa, Elci Latif
Pamukkale University, Faculty of Arts and Science, Department of Chemistry, 20020 Denizli, Turkey.
Talanta. 2009 Oct 15;79(5):1287-91. doi: 10.1016/j.talanta.2009.05.046. Epub 2009 Jun 6.
The present paper proposes a preconcentration procedure for phosphorus determination by using ultraviolet-visible spectrophotometer. It is based on the formation of phosphomolybdate and its reduction to molybdenum blue. Phosphorus extraction as phosphomolybdenum blue complex was performed onto Amberlite XAD-4. The optimization step was carried out using two-level full factorial design. Three variables (resin amount, sample volume, flow rate) were regarded as factors in the optimization. The relative standard deviation was 2% at 0.08 microg mL(-1). The limit of detection was found to be 2.23 microg L(-1) (N=15). The proposed solid-phase extraction procedure was applied to phosphorus in some fruit leaves, natural waters, and a standard reference material (SRM 1515 apple leaves).
本文提出了一种使用紫外可见分光光度计测定磷的预浓缩方法。该方法基于磷钼酸盐的形成及其还原为钼蓝。以磷钼蓝络合物形式将磷萃取到Amberlite XAD - 4上。优化步骤采用二级全因子设计进行。在优化过程中，将三个变量（树脂量、样品体积、流速）视为因素。在0.08 μg mL⁻¹时相对标准偏差为2%。检测限为2.23 μg L⁻¹（N = 15）。所提出的固相萃取方法应用于一些果树叶、天然水和一种标准参考物质（SRM 1515苹果叶）中的磷测定。
