Sargent Rachel L, Craig Fiona E, Swerdlow Steven H
Department of Pathology, Division of Hematopathology, University of Pittsburgh School of Medicine Pittsburgh, PA, USA.
Int J Clin Exp Pathol. 2009 Jun 16;2(6):574-82.
Chronic lymphocytic leukemia (CLL) was previously considered a uniform disease characterized by autonomous over-expression of bcl-2. Recently the pathogenic role of bcl-2 has been questioned and attention has turned to prognostic subtypes of CLL differing in CD38 and ZAP-70 expression. However, the relationship between bcl-2 and CD38 or ZAP-70 expression remains uncertain and was investigated using flow cytometric immunophenotyping of 50 CLL specimens. CLL cells were consistently bcl-2 positive but varied in expression level: mean fluorescence intensity (MFI) 45-152. Although there was no significant difference in bcl-2 expression between CD38 or ZAP-70 positive and negative specimens, an inverse correlation was identified between percentage of CD38 positive B-cells and bcl-2 MFI when all (p<0.03, r(2)=0.10) and peripheral blood (p<0.004, r(2)=0.27) samples were analyzed. While bcl-2 levels do not appear to be a major discriminator between indolent and more aggressive subtypes of CLL, CD38 and bcl-2 expression appear to be interrelated.
慢性淋巴细胞白血病（CLL）以前被认为是一种单一疾病，其特征为bcl-2的自主性过度表达。最近，bcl-2的致病作用受到质疑，人们的注意力转向了CD38和ZAP-70表达不同的CLL预后亚型。然而，bcl-2与CD38或ZAP-70表达之间的关系仍不确定，因此我们使用流式细胞术免疫表型分析对50例CLL标本进行了研究。CLL细胞始终呈bcl-2阳性，但表达水平有所不同：平均荧光强度（MFI）为45 - 152。虽然CD38或ZAP-70阳性和阴性标本之间的bcl-2表达没有显著差异，但在分析所有样本（p<0.03，r(2)=0.10）和外周血样本（p<0.004，r(2)=0.27）时发现，CD38阳性B细胞百分比与bcl-2 MFI呈负相关。虽然bcl-2水平似乎不是惰性和侵袭性更强的CLL亚型之间的主要区分因素，但CD38和bcl-2表达似乎相互关联。
