Zivkovic Zorica, Blouin Edmour F, Manzano-Roman Raúl, Almazán Consuelo, Naranjo Victoria, Massung Robert F, Jongejan Frans, Kocan Katherine M, de la Fuente José
Utrecht Centre for Tick-Borne Diseases (UCTD), Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Comp Funct Genomics. 2009;2009:705034. doi: 10.1155/2009/705034. Epub 2009 Jul 15.
The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells. Recently, we showed that A. marginale affects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile in A. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile in Ixodes scapularis ticks and cultured ISE6 cells in response to infection with A. phagocypthilum and to compare tick gene expression responses in A. phagocytophilum- and A. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale. These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.
无形体属（立克次氏体目：无形体科）包括专性蜱传播的细胞内生物，嗜吞噬细胞无形体和边缘无形体，它们在脊椎动物和蜱宿主细胞中均可繁殖。最近，我们发现边缘无形体可影响蜱虫中与蜱虫存活以及病原体感染和繁殖相关的基因表达。然而，目前对于嗜吞噬细胞无形体感染的蜱细胞中的基因表达谱了解甚少。本研究的目的是通过微阵列和实时逆转录聚合酶链反应分析，来表征肩突硬蜱和培养的ISE6细胞对嗜吞噬细胞无形体感染的蜱基因表达谱，并比较嗜吞噬细胞无形体和边缘无形体感染的蜱细胞中蜱基因的表达反应。这些研究结果证明了嗜吞噬细胞无形体对蜱基因表达的调节作用，并提供了证据表明在嗜吞噬细胞无形体和边缘无形体感染的蜱细胞中存在不同的基因表达反应。无形体与蜱相互作用的这些差异可能反映了病原体在蜱细胞中生命周期的差异。
