肠炎沙门氏菌铜锌超氧化物歧化酶还原形式的核磁共振归属
NMR assignment of reduced form of copper, zinc superoxide dismutase from Salmonella enterica.
作者信息
Jiménez Beatriz, Mori Mirko, Battistoni Andrea, Sette Marco, Piccioli Mario
机构信息
Magnetic Resonance Center (CERM) and Department of Chemistry, University of Florence, Via L Sacconi 6, Sesto Fiorentino, Florence 50019, Italy.
出版信息
Biomol NMR Assign. 2007 Jul;1(1):65-7. doi: 10.1007/s12104-007-9017-0. Epub 2007 May 22.
Almost complete assignment (97%) of NMR resonances was obtained for the reduced, Cu(I), form of prokaryotic CuZnSOD from Salmonella enterica. 13C direct detection was used to complement the standard bouquet of 1H detected triple resonance experiments and contributed to the identification of proline backbone resonances and to side chains assignments of Asx, Glx and aromatic rings. This is the only complete assignment available for monomer SOD from prokaryotic organisms.
对于来自肠炎沙门氏菌的原核铜锌超氧化物歧化酶（CuZnSOD）的还原态Cu(I)形式，几乎完成了97%的核磁共振（NMR）共振峰归属。采用13C直接检测来补充标准的1H检测三重共振实验组合，并有助于脯氨酸主链共振峰的识别以及天冬氨酸/天冬酰胺（Asx）、谷氨酸/谷氨酰胺（Glx）侧链和芳香环的归属。这是原核生物单体超氧化物歧化酶唯一可用的完整归属。
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