Perez Daniel R, Wider Gerhard
Institute of Molecular Biology and Biophysics, ETH Zurich, 8093, Zurich, Switzerland.
Biomol NMR Assign. 2009 Jun;3(1):133-6. doi: 10.1007/s12104-009-9158-4. Epub 2009 Apr 7.
Acyl carrier proteins participate in the synthesis of fatty acids. Here we report the NMR resonances assignment of the acyl carrier protein domain of the Saccharomyces cerevisiae fatty acid synthase which corresponds to the fragment 138A-302L in the primary structure. The assignment will allow performing NMR studies with the aim to investigate the intrinsic dynamics of this protein, and to study the structural changes upon apo-holo transformation in order to unveil the mechanism of binding of the growing acyl chain.
酰基载体蛋白参与脂肪酸的合成。在此我们报告酿酒酵母脂肪酸合酶酰基载体蛋白结构域的核磁共振(NMR)共振峰归属,该结构域在一级结构中对应于138A - 302L片段。该归属将有助于开展NMR研究,旨在探究该蛋白的内在动力学,并研究脱辅基 - 全酶转变时的结构变化,以揭示生长中的酰基链的结合机制。