从与磺酸盐羧酸酯基异戊二烯/环氧乙烷嵌段共聚物形成的复合物中，溶菌酶具有结构和活性的恢复，实现持续和延长的释放。

Sustained and extended release with structural and activity recovery of lysozyme from complexes with sodium (sulfamate carboxylate) isoprene/ethylene oxide block copolymer.

机构信息

Key Laboratory of Molecular Engineering of Polymers, and Department of Macromolecular Science, Fudan University, Shanghai 200433, China.

出版信息

Macromol Biosci. 2010 Feb 11;10(2):139-46. doi: 10.1002/mabi.200900186.

DOI:10.1002/mabi.200900186

PMID:19637151

Abstract

The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residues of lysozyme are exposed to bind with SCIEO. The complexation leads to loss of most of the lysozyme activity. In the presence of NaCl, lysozyme can be released from the complexes. The released lysozyme molecules recover their native structure and activity completely. In the condition of physiological pH and ionic strength, a sustained and extended release of lysozyme was achieved.

摘要

在 pH = 7.4 下研究了溶菌酶与磺酸盐羧酸酯异戊二烯/环氧乙烷（SCIEO）的络合作用，以及在 NaCl 存在下溶菌酶从复合物中的释放。通过静电和疏水相互作用，溶菌酶和 SCIEO 形成稳定的复合纳米颗粒。络合作用部分破坏了溶菌酶的结构。溶菌酶的一些疏水性残基暴露出来与 SCIEO 结合。这种络合作用导致溶菌酶失去大部分活性。在 NaCl 的存在下，溶菌酶可以从复合物中释放出来。释放的溶菌酶分子完全恢复其天然结构和活性。在生理 pH 值和离子强度条件下，实现了溶菌酶的持续和延长释放。