[从老虎分离的猫泛白细胞减少症病毒灭活疫苗的制备及初步应用]

[Preparation of the vaccine with inactivated Feline Panleukopenia Virus isolated from tiger and the preliminary application].

作者信息

Yu Yali, Zhou Ming, Zhang Jin, Hua Yuping, Wang Ligang, Liu Yinan, Liu Dan, Xia Xianzhu

机构信息

College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China.

出版信息

Wei Sheng Wu Xue Bao. 2009 May;49(5):643-7.

PMID:19637573

Abstract

OBJECTIVE

To prepare a vaccine with inactivated Feline Panleukopenia Virus (FPV) isolated from Siberian tigers and to evaluate its immunological effect.

METHODS

FPV-HLJ, an FPV strain previously isolated from Siberian tiger in our lab was used to inoculate cat kidney cell line F81 with dose 1/10(v/v) using the synchronizing inoculation method. Inoculated F81 cell line was cultured at 37 degrees C and collected when cytopathic effect was up to 75%. Viral suspension was inactivated by using formaldehyde for 24 h and inactive vaccine preapred by adding aluminium hydroxide gel as adjuvant to the suspension. The inactive vaccine was applied to 2-month-old kitten tigers of hypodermically after protection effect was proved in 2-month-old nonimmunized cats by using the same vaccination procedure.

RESULTS

Antibody level in vaccinated cats revealed an increasing trend that the valence of antibody reached 1:1024-2048 after three times of vaccination. All vaccinated cats survived challenges of virulent FPV virus. The valence of antibody reached 1:1024 in most vaccinated tigers 15 days after the third vaccination.

CONCLUSION

The results indicated that the inactivated vaccine can produce immunoprotection for tigers.

摘要

目的

制备一种用从东北虎分离出的猫泛白细胞减少症病毒（FPV）灭活制成的疫苗，并评估其免疫效果。

方法

使用本实验室先前从东北虎分离出的FPV毒株FPV-HLJ，采用同步接种法以1/10（v/v）的剂量接种猫肾细胞系F81。接种后的F81细胞系在37℃培养，当细胞病变效应达到75%时收集。病毒悬液用甲醛灭活24小时，并通过向悬液中添加氢氧化铝凝胶作为佐剂制备灭活疫苗。在通过相同的接种程序在2月龄未免疫的猫中证明保护效果后，将灭活疫苗皮下注射给2月龄的幼虎。