Wang Sijun, Qi Wenbao, Xu Chenggang, Luo Kaijian, Zhang Guihong, Ye Hejia, Liao Ming
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Wei Sheng Wu Xue Bao. 2009 May;49(5):653-7.
To evaluate the immunity of the adenovirus recombinant rAd-HA-GFP encoding an H3N2 swine influenza virus hemagglutinin.
Thee groups of 6-week-old pigs (5 pigs per group) were vaccinated intramuscularly with the recombinant, one group was immunized with 10(-8) TCID50 recombinant adenovirus rAd-HA-GFP, the other groups were vaccinated with 2 x 10(-8) TCID50 and 4 x 10(-8) TCID50. A control groups (5 pigs) were vaccinated with recombinant adenovirus rAd-GFP. Virus-specific hemagglutination-inhibition (HI) antibody was detected by 2-6 weeks post vaccination. Compared the difference of vaccinated intramuscularly, intragastric administration and intranasal inoculation, three groups of different HI antibody pigs and one control group were challenged with a virulent H3N2 field virus.
The results showed that pigs in the groups given higher immunizing dose were developed higher levels HI antibody. All of vaccinated intramuscularly, intragastric administration and intranasal inoculation could produce HI antibody, but the titer of vaccinated intramuscularly was higher significantly (P < 0.01). Three groups of pigs (5 pigs per group) which HI titers were 1:80, 1:160, 1:320 respectively were challenged with a virulent H3N2 field virus, include the negative control group. The immunization efficacy was evaluated by clinical signs, the rate of virus isolation and HI titer. It suggested that immunological response induced by rAd-HA-GFP could resist attack of SIV effectively.
The adenovirus vaccines rAd-HA-GFP are efficacious for SIV and have the additional advantage over commercial vaccines that suckling piglets have no pre-existing maternally-derived antibody to block early life vaccination.
评估编码H3N2猪流感病毒血凝素的腺病毒重组体rAd-HA-GFP的免疫性。
将三组6周龄仔猪（每组5头）肌肉注射接种该重组体，一组用10(-8) TCID50重组腺病毒rAd-HA-GFP免疫，其他两组分别用2×10(-8) TCID50和4×10(-8) TCID50接种。一组对照组（5头猪）用重组腺病毒rAd-GFP接种。在接种后2至6周检测病毒特异性血凝抑制（HI）抗体。比较肌肉注射、胃内给药和鼻内接种的差异，将三组不同HI抗体水平的猪和一组对照组用强毒H3N2野毒攻击。
结果显示，给予较高免疫剂量组的猪产生了更高水平的HI抗体。肌肉注射、胃内给药和鼻内接种均可产生HI抗体，但肌肉注射组的抗体滴度显著更高（P<0.01）。将HI滴度分别为1:80、1:160、1:320的三组猪（每组5头），包括阴性对照组，用强毒H3N2野毒攻击。通过临床症状、病毒分离率和HI滴度评估免疫效果。结果表明，rAd-HA-GFP诱导的免疫反应可有效抵抗猪流感病毒的攻击。
腺病毒疫苗rAd-HA-GFP对猪流感病毒有效，且与商业疫苗相比具有额外优势，即哺乳仔猪不存在预先存在的母源抗体来阻断早期疫苗接种。
