Lu Changyong, Shi Chunlei, Zhang Chunxiu, Chen Jing, Shi Xianming
Department of Food Science and Bor Luh Food Safety Center, School ofAgriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Apr;25(4):554-9.
We developed single base extension-tags (SBE-tags) microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157:H7 and Campylobacter jejuni. With specific PCR primers identified and integrated for eight food-borne pathogens, target sequences were amplified and purified as template DNA of single base extension-tags reaction. The products were hybridized to microarrays and scanned for fluorescence intensity. The experiment showed a specific and simultaneous detection of eight food-borne pathogens. The system limits is 0.1 pg for a genomic DNA and 5x10(2) CFU/mL for Salmonella typhimurium cultures. The single base extension-tags assay can be used to detect food-borne pathogens rapidly and accurately with a high sensitivity, and provide an efficient way for diagnosis and control of disease caused by food-borne pathogens.
我们开发了单碱基延伸标签（SBE标签）微阵列来检测8种常见的食源性病原体，包括金黄色葡萄球菌、副溶血性弧菌、单核细胞增生李斯特菌、沙门氏菌、阪崎肠杆菌、志贺氏菌、大肠杆菌O157:H7和空肠弯曲菌。通过鉴定并整合8种食源性病原体的特异性PCR引物，扩增并纯化目标序列作为单碱基延伸标签反应的模板DNA。将产物与微阵列杂交并扫描荧光强度。实验表明该方法能够特异性且同时检测8种食源性病原体。该系统对基因组DNA的检测限为0.1 pg，对鼠伤寒沙门氏菌培养物的检测限为5×10² CFU/mL。单碱基延伸标签检测法可用于快速、准确且高灵敏度地检测食源性病原体，为食源性病原体所致疾病的诊断和控制提供了一种有效的方法。
