Daumas Stéphanie, Ceccom Johnatan, Halley Hélène, Francés Bernard, Lassalle Jean-Michel
Université de Toulouse, France.
Learn Mem. 2009 Jul 28;16(8):504-7. doi: 10.1101/lm.1418309. Print 2009 Aug.
Elucidating the functional properties of the dentate gyrus (DG), CA3, and CA1 areas is critical for understanding the role of the dorsal hippocampus in contextual fear memory processing. In order to specifically disrupt various hippocampal inputs, we used region-specific infusions of DCG-IV, the metabotropic glutamate receptor agonist, which selectively disrupts entorhinal outputs as well as mossy fiber transmission in the hippocampus. The consequences of these injections were studied using a contextual fear conditioning (CFC) paradigm. Selective contextual memory impairment was observed in DG- and CA3-, but not in CA1-treated mice. Our results emphasize the major role played by the DG and CA3 areas in the early phases of contextual memory processing, particularly during the acquisition and early consolidation phases of CFC.
阐明齿状回（DG）、CA3和CA1区域的功能特性对于理解背侧海马体在情境恐惧记忆处理中的作用至关重要。为了特异性地破坏各种海马体输入，我们使用了代谢型谷氨酸受体激动剂DCG-IV进行区域特异性注射，该激动剂可选择性地破坏海马体中的内嗅输出以及苔藓纤维传递。我们使用情境恐惧条件反射（CFC）范式研究了这些注射的后果。在注射到DG和CA3区域的小鼠中观察到了选择性情境记忆损伤，但在注射到CA1区域的小鼠中未观察到。我们的结果强调了DG和CA3区域在情境记忆处理早期阶段，特别是在CFC的获取和早期巩固阶段所起的主要作用。
