Kong H J, Hong G-E, Cho H K, Nam B-H, Kim Y-O, Kim W-J, Lee S-J, Kim K-K
Biotechnology Research Institute, National Fisheries Research and Development Institute, Sirang-ri, Gijang-up, Gijang-gun, Busan, Republic of Korea.
J Appl Genet. 2009;50(3):245-50. doi: 10.1007/BF03195678.
We isolated and characterized the profilin (FcPFN) cDNA from hemocytes of Fenneropenaeus chinensis, a unique shrimp species from the Yellow Sea. The FcPFN cDNA consists of 830 bp and encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.06. The deduced amino acid sequence of FcPFN shows 36% and 90% amino acid sequence identity to the profilin genes of Pacific white shrimp Litopenaeus vannamei and black tiger shrimp Penaeus monodon, respectively. The FcPFN mRNA was highly expressed in hemocytes and hepatopancreas and moderately in muscle of normal shrimp. The higher expression of FcPFN mRNA is observed in shrimp infected with the white spot syndrome virus (WSSV), which is a major concern in all shrimp-growing regions of the world. These results suggest a potential role for FcPFN in viral host defense mechanisms.
我们从中国对虾（一种来自黄海的独特虾类）的血细胞中分离并鉴定了肌动蛋白结合蛋白（FcPFN）的cDNA。FcPFN的cDNA由830个碱基对组成，编码一个含有125个氨基酸的多肽，预测的等电点为5.06。FcPFN推导的氨基酸序列与凡纳滨对虾和斑节对虾的肌动蛋白结合蛋白基因的氨基酸序列一致性分别为36%和90%。在正常对虾中，FcPFN的mRNA在血细胞和肝胰腺中高度表达，在肌肉中适度表达。在感染了白斑综合征病毒（WSSV）的对虾中观察到FcPFN的mRNA表达较高，而WSSV是全球所有对虾养殖区的主要关注点。这些结果表明FcPFN在病毒宿主防御机制中可能发挥作用。
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