在理解裂殖酵母胞质分裂机制方面取得的进展。
Progress towards understanding the mechanism of cytokinesis in fission yeast.
作者信息
Pollard Thomas D
机构信息
Department of Molecular, Cellular and Developmental Biology, Cell Biology, Yale University, New Haven, CT 06520-8103, USA.
出版信息
Biochem Soc Trans. 2008 Jun;36(Pt 3):425-30. doi: 10.1042/BST0360425.
Abstract
We use fission yeast to study the molecular mechanism of cytokinesis. We benefit from a long history in genetic analysis of the cell cycle in fission yeast, which provided the most complete inventory of cytokinesis proteins. We used fluorescence microscopy of proteins tagged with fluorescent proteins to establish the temporal and spatial pathway for the assembly and constriction of the contractile ring. We combined biochemical analysis of purified proteins (myosin-II, profilin, formin Cdc12p and cofilin), observations of fluorescent fusion proteins in live cells and mathematical modelling to formulate and test a simple hypothesis for the assembly of the contractile ring. This model involves the formation of 65 nodes containing myosin-II and formin Cdc12p around the equator of the cell. As a cell enters anaphase, actin filaments grow from formin Cdc12p in these nodes. Myosin captures actin filaments from adjacent nodes and pulls intermittently to condense the nodes into a contractile ring.
摘要
我们利用裂殖酵母来研究胞质分裂的分子机制。我们受益于裂殖酵母细胞周期遗传分析的悠久历史，该分析提供了最完整的胞质分裂蛋白清单。我们使用荧光蛋白标记的蛋白质的荧光显微镜来建立收缩环组装和收缩的时空途径。我们结合了对纯化蛋白（肌球蛋白-II、丝切蛋白、formin Cdc12p和cofilin）的生化分析、活细胞中荧光融合蛋白的观察以及数学建模，以制定和测试一个关于收缩环组装的简单假设。该模型涉及在细胞赤道周围形成65个包含肌球蛋白-II和formin Cdc12p的节点。当细胞进入后期时，肌动蛋白丝从这些节点中的formin Cdc12p生长出来。肌球蛋白从相邻节点捕获肌动蛋白丝并间歇性拉动，将节点浓缩成收缩环。
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