胶原酶对雏鸡基底乳头毛细胞纤毛连接的影响。

Influence of collagenase on tip links in hair cells of the chick basilar papilla.

作者信息

Pickles J O, Brix J, Manley G A

机构信息

Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Australia.

出版信息

Hear Res. 1990 Dec;50(1-2):139-43. doi: 10.1016/0378-5955(90)90040-v.

DOI:10.1016/0378-5955(90)90040-v

PMID:1963885

Abstract

Chick basilar papillae were incubated in collagenase, using concentrations previously employed for the isolation of viable hair cells. When assessed by scanning electron microscopy, the hair bundles had a normal conformation, with no loss of tip links compared with control incubations. The results suggest that collagenase dose not destroy the integrity of structures on the apical surface of hair cells, and that tip links are not composed of collagen. The results are in agreement with the hypothesis that tip links are involved in mechanotransduction.

摘要

将鸡的基底乳头置于胶原酶中孵育，使用先前用于分离活毛细胞的浓度。通过扫描电子显微镜评估时，与对照孵育相比，毛束具有正常的构象，尖端连接没有损失。结果表明，胶原酶不会破坏毛细胞顶端表面结构的完整性，并且尖端连接不是由胶原蛋白组成。这些结果与尖端连接参与机械转导的假设一致。