细胞外基质金属蛋白酶诱导剂（EMMPRIN）在健康和炎症状态下的人牙龈中的表达
Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in healthy and inflamed human gingival.
作者信息
Xiang Junbo, Cao Zhengguo, Dong Weili, Li Chengzhang
机构信息
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
出版信息
Quintessence Int. 2009 Sep;40(8):683-90.
OBJECTIVE
To evaluate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in healthy and inflamed human gingiva.
METHOD AND MATERIALS
Tissue samples from 9 healthy specimens and 21 specimens with chronic periodontitis were collected. The expression of EMMPRIN protein was detected with immunohistochemical staining and Western blot analysis.
RESULTS
EMMPRIN was mainly localized in keratinocytes of healthy human gingival tissues; in inflamed gingival tissues, EMMPRIN protein could be detected in keratinocytes, fibroblasts, and inflammatory cells. In addition, EMMPRIN expression level in the inflamed gingiva was increased dramatically compared to that in healthy tissue (P < .05). Using Western blot analysis, EMMPRIN protein was also found to express in both healthy and inflamed gingiva, and the level of EMMPRIN in inflamed gingiva was significantly higher than that in healthy control subjects (P < .05).
CONCLUSION
EMMPRIN might be involved in the physiologic and pathologic states of periodontal tissues.
目的
评估细胞外基质金属蛋白酶诱导剂（EMMPRIN）在健康和炎症状态下的人牙龈组织中的表达情况。
方法与材料
收集9例健康样本和21例慢性牙周炎样本的组织。采用免疫组织化学染色和蛋白质印迹分析检测EMMPRIN蛋白的表达。
结果
EMMPRIN主要定位于健康人牙龈组织的角质形成细胞；在炎症牙龈组织中，角质形成细胞、成纤维细胞和炎症细胞中均可检测到EMMPRIN蛋白。此外，与健康组织相比，炎症牙龈中EMMPRIN的表达水平显著升高（P <.05）。通过蛋白质印迹分析还发现，健康和炎症牙龈中均有EMMPRIN蛋白表达，且炎症牙龈中EMMPRIN水平显著高于健康对照受试者（P <.05）。
结论
EMMPRIN可能参与牙周组织的生理和病理状态。
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