Dental Department, Cathay General Hospital, Taipei, Taiwan.
Med Biol Eng Comput. 2009 Dec;47(12):1273-9. doi: 10.1007/s11517-009-0512-6.
Recently, the term tensotaxis was proposed to describe the phenomenon that tensile stress or strain affects cell migration. Even so, less attention has been paid to the effects of compressive stress on cell behavior. In this study, by using an injection-molded method combined with photoelastic technology, we developed residual stress gradient-controlled poly-L-lactide discs. After culturing NIH-3T3 fibroblasts on the stress gradient substrate, the cell distributions for high- and low-stress regions were measured and compared. Our results showed that there were significantly more cells in the low-compressive stress region relative to their high-stress analogs (p < 0.05). In addition, NIH-3T3 fibroblasts in the low-compressive stress region expressed more abundant extensive filopodia. These findings provide greater insight into the interaction between cells and substrates, and could serve as a useful reference for connective tissue development and repair.
最近，有人提出了“张力趋化”这一术语来描述张力或应变对细胞迁移的影响。即便如此，人们对压缩应力对细胞行为的影响关注较少。在这项研究中，我们采用注塑成型法结合光弹技术，制备了残余应力梯度可控的聚 L-乳酸盘。在将 NIH-3T3 成纤维细胞培养在具有应力梯度的基底上之后，我们测量并比较了高、低应区的细胞分布。结果表明，低压缩应区的细胞数量明显多于高应区（p < 0.05）。此外，低压缩应区的 NIH-3T3 成纤维细胞表达了更丰富的广泛丝状伪足。这些发现为细胞与基底之间的相互作用提供了更深入的了解，并可能为结缔组织的发育和修复提供有益的参考。
