Labes G, Simon R
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
Plasmid. 1990 Nov;24(3):235-9. doi: 10.1016/0147-619x(90)90007-y.
In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restriction and hybridization experiments revealed that identical ISp elements could be isolated from different R. meliloti strains and that one of these is similar to an insertion sequence found previously in R. meliloti 2011.
为了筛选能够促进侧翼基因转录的插入序列（ISp元件），构建了三种源自可移动质粒RSF1010的载体。利用无启动子的抗生素抗性基因，从大肠杆菌和苜蓿根瘤菌中分离出长度在0.75至2.9 kb之间的ISp元件。限制性内切酶分析和杂交实验表明，相同的ISp元件可从不同的苜蓿根瘤菌菌株中分离得到，且其中一个与先前在苜蓿根瘤菌2011中发现的插入序列相似。
