Kapková Petra
Department of Parmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
Rapid Commun Mass Spectrom. 2009 Sep;23(17):2775-84. doi: 10.1002/rcm.4187.
A derivatization method for mass spectrometric analysis of oligosaccharides is presented. Small saccharides, complex, high-mannose-type oligosaccharides and oligosaccharides released from hen ovalbumin were converted into their biotin derivatives by incubating them with biotinamidocaproyl hydrazide (BACH). Improved sensitivity of mass spectrometric analysis of labeled glycans in comparison with their natural counterparts was achieved after derivatization. The labeling reagent contains a biotin handle at one end and a hydrazide group at the other. Hence, the key feature of biotinylated sugars is that in addition to their usefulness in functional studies (e.g. analysis of the interaction between lectins and biotin-derivatized oligosaccharides) they might be utilized also for structural analysis of oligosaccharides. Mass spectrometric studies were performed by matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization mass spectrometry.
本文介绍了一种用于寡糖质谱分析的衍生化方法。通过将小糖类、复杂的高甘露糖型寡糖以及从鸡卵清蛋白中释放出的寡糖与生物素氨甲酰肼（BACH）孵育，将它们转化为生物素衍生物。衍生化后，与天然对应物相比，标记聚糖的质谱分析灵敏度得到了提高。标记试剂一端含有生物素柄，另一端含有酰肼基团。因此，生物素化糖的关键特性在于，除了在功能研究中有用（例如分析凝集素与生物素衍生化寡糖之间的相互作用）外，它们还可用于寡糖的结构分析。质谱研究通过基质辅助激光解吸/电离飞行时间质谱和电喷雾电离质谱进行。
