非酶促的依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的2,6-二氯酚靛酚还原反应。
Nonenzymatic NADPH-dependent reduction of 2,6-dichlorophenol-indophenol.
作者信息
Dupuy C, Kaniewski J, Ohayon R, Dème D, Virion A, Pommier J
机构信息
U.96 INSERM, Unité de Recherche sur la Glande Thyroïde et la Régulation Hormonale, Le Kremlin-Bicêtre, France.
出版信息
Anal Biochem. 1990 Nov 15;191(1):16-20. doi: 10.1016/0003-2697(90)90379-n.
The reduction of 2,6-dichloroindophenol (DCIP) by direct interaction with NADPH was studied. The results indicate that reduction proceeds via a direct electron transfer from NADPH to DCIP, with no oxygen consumption, and a rate constant of k = 4.69 M-1.s-1. The reduced DCIP can rapidly transfer its electrons to potassium ferricyanide (K3Fe(CN)6) or ferricytochrome c, but not to nitro blue tetrazolium. Superoxide dismutase inhibits DCIP reduction in an oxygen-dependent manner by favoring the reoxidation of the reduced DCIP. We therefore conclude DCIP is not suitable for detecting O2- when the nucleotides NADH or NADPH are present.
研究了通过与NADPH直接相互作用对2,6 - 二氯靛酚（DCIP）的还原作用。结果表明，还原过程是通过从NADPH到DCIP的直接电子转移进行的，不消耗氧气，速率常数k = 4.69 M-1·s-1。还原后的DCIP可以迅速将其电子转移到铁氰化钾（K3Fe(CN)6）或细胞色素c，但不能转移到硝基蓝四氮唑。超氧化物歧化酶通过促进还原型DCIP的再氧化，以氧依赖的方式抑制DCIP的还原。因此，我们得出结论，当存在核苷酸NADH或NADPH时，DCIP不适用于检测O2-。
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