大鼠肝微粒体组分中一些受体与NADPH特异性黄素蛋白形成的超氧阴离子自由基的相互作用。
Interactions of some acceptors with superoxide anion radicals formed by the NADPH-specific flavoprotein in rat liver microsomal fractions.
作者信息
Mishin V, Pokrovsky A, Lyakhovich V V
出版信息
Biochem J. 1976 Feb 15;154(2):307-10. doi: 10.1042/bj1540307.
Abstract
In rat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (O2-), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific flavoprotein only directly. Nitro Blue Tetrazolium was reduced both by O2- and by the direct acceptance of electrons. Elevation of pH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2--dependent pathway. From the values of the kinetic constants for interaction of adrenaline and Nitro Blue Tetrazolium with NADPH-specific flavoprotein, the rates of generation of O2- in rat liver microsomal fraction were determined.
摘要
在大鼠肝脏微粒体组分中，肾上腺素的氧化是由超氧阴离子自由基（O2-）介导的，而细胞色素c、2,6-二氯酚靛酚和铁氰化物仅直接从NADPH特异性黄素蛋白接受电子。硝基蓝四唑既可以被O2-还原，也可以直接接受电子而被还原。提高pH值和添加甲萘醌会使硝基蓝四唑的还原向依赖O2-的途径转变。根据肾上腺素和硝基蓝四唑与NADPH特异性黄素蛋白相互作用的动力学常数，测定了大鼠肝脏微粒体组分中O2-的生成速率。
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