正常人类受试者外周血单个核细胞中1,25(OH)₂D₃受体的性别和年龄分布

Sex and age distribution of 1,25(OH)2D3 receptors in peripheral blood mononuclear cells from normal human subjects.

作者信息

Olmos J M, Amado J A, Riancho J A, Albájar M, González-Macías J

机构信息

Departamento de Medicina Interna, Hospital Nacional Marqués de Valdecilla, Facultad de Medicina, Santander, Spain.

出版信息

Bone. 1990;11(6):407-9. doi: 10.1016/8756-3282(90)90135-l.

DOI:10.1016/8756-3282(90)90135-l

PMID:1964061

Abstract

Specific receptors for 1,25 Dihydroxyvitamin D3 have been described in human peripheral blood mononuclear cells (PBMC). We have tried to find out whether these receptors could show any difference in sex or age distribution. Twenty two healthy men aged 21-66 yr (mean +/- SD 41.0 +/- 13.6) and nineteen healthy women aged 22-60 yr (38.9 +/- 13.9) have been studied. The mean dissociation constant (Kd) was similar in both sexes (1.35 +/- 0.70 x 10(-10) M in males, 1.13 +/- 0.66 x 10(-10) M in females), but the concentration of binding sites (Nmax) was significantly lower in females (2.32 +/- 0.92 fmol/10(7) PBMC vs 4.43 +/- 1.38 fmol/10(7) PBMC in males; p = 0.0001). Neither Kd nor Nmax were significantly correlated with age. No difference was found between pre and postmenopausal women. Further studies are needed to elucidate if this sex difference in PBMC receptors for 1.25 Dihydroxyvitamin D3 is of any pathophysiological relevance.

摘要

1,25-二羟基维生素D3的特异性受体已在人外周血单个核细胞（PBMC）中被描述。我们试图弄清楚这些受体在性别或年龄分布上是否存在差异。研究了22名年龄在21 - 66岁（平均±标准差41.0±13.6）的健康男性和19名年龄在22 - 60岁（38.9±13.9）的健康女性。两性的平均解离常数（Kd）相似（男性为1.35±0.70×10⁻¹⁰M，女性为1.13±0.66×10⁻¹⁰M），但女性的结合位点浓度（Nmax）显著较低（女性为2.32±0.92 fmol/10⁷PBMC，男性为4.43±1.38 fmol/10⁷PBMC；p = 0.0001）。Kd和Nmax均与年龄无显著相关性。绝经前和绝经后女性之间未发现差异。需要进一步研究以阐明PBMC中1,25-二羟基维生素D3受体的这种性别差异是否具有任何病理生理相关性。