Huang Shengchang, Wang Jianle, Liu Sheng, Li Yueying, Hu Jianjun, Kou Zhaohui, Zhang Yu, Sun Xiaofang, Gao Shaorong
National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, PR China.
Differentiation. 2009 Sep-Oct;78(2-3):151-8. doi: 10.1016/j.diff.2009.06.006. Epub 2009 Jul 28.
Recent advances have demonstrated that the differentiated somatic cells could be reprogrammed into pluripotent state. Consequently, the reprogrammed somatic cells recapitulate the capacity to differentiate into specific cell lineages under appropriate culture conditions, which provides unlimited cell sources for cell transplantation-based therapy. In the present study, testicular Sertoli cells were successfully reprogrammed into pluripotent stem cells through somatic cell nuclear transfer (SCNT). Hematopoietic differentiation potential of the reprogrammed somatic cells was investigated in parallel to fertilization-derived ES (F-ES) cells. Our results demonstrated that the reprogrammed Sertoli cells (NT-ES) could efficiently differentiate into hematopoietic embryoid bodies (EBs). The hematopoietic-related genes including FLK-1, Bmp4, Runx1, etc. were dynamically expressed during the differentiation of the reprogrammed somatic cells in vitro. Transplantation of these differentiated reprogrammed cells into the bone marrow of irradiated mice could allow differentiation into different functional hematopoietic lineages in vivo. Moreover, blast-colony-forming cells (BL-CFCs) could be generated from both NT-ES and F-ES cells with similar efficiency in vitro. Our study indicates that the reprogrammed somatic cells possess the equivalent potency as F-ES cells in differentiating into functional hematopoietic cells.
最近的研究进展表明，已分化的体细胞可以被重编程为多能状态。因此，重编程后的体细胞在适当的培养条件下具备分化为特定细胞谱系的能力，这为基于细胞移植的治疗提供了无限的细胞来源。在本研究中，通过体细胞核移植（SCNT）成功地将睾丸支持细胞重编程为多能干细胞。同时，对重编程体细胞与受精来源的胚胎干细胞（F-ES细胞）的造血分化潜能进行了研究。我们的结果表明，重编程后的支持细胞（NT-ES）能够有效地分化为造血类胚体（EBs）。在体外重编程体细胞分化过程中，包括FLK-1、Bmp4、Runx1等在内的造血相关基因呈动态表达。将这些分化后的重编程细胞移植到受辐照小鼠的骨髓中，它们在体内能够分化为不同功能的造血谱系。此外，NT-ES细胞和F-ES细胞在体外能够以相似的效率产生爆式集落形成细胞（BL-CFCs）。我们的研究表明，重编程体细胞在分化为功能性造血细胞方面具有与F-ES细胞相当的潜能。
