Trusk T C, Kaboord W S, Wong-Riley M T
Department of Anatomy and Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.
Vis Neurosci. 1990 Mar;4(3):185-204. doi: 10.1017/s0952523800003345.
The laminar structure and cellular distribution of cytochrome-oxidase (CO) reactivity in supragranular puffs of striate cortex was examined in adult macaque monkeys surviving various periods of monocular enucleation, lid suture, and retinal impulse blockage with tetrodotoxin (TTX). Enucleation and TTX produced a rapid and severe loss in the size of the CO reactive region in puffs dominated by the removed or treated eye compared to slower and less marked reductions obtained in deprived puffs of lid-sutured monkeys. In all deprived animals, the cross-sectional areas of deprived puffs decreased most rapidly in the upper layers (2 and 3A). In long-term enucleated (60 wks) and TTX-treated (4 wks) monkeys, puff area was severely reduced in layer 3B, while reactivity in layer 3B appeared partially spared in lid-sutured monkeys. The density of the CO reaction product was significantly and evenly reduced throughout deprived puffs for all of the monkeys examined; however, this decrease was less severe in adult monkeys lid-sutured for 11 wks. Although no evidence for cell loss was obtained, all three forms of visual deprivation led to lower counts of neuronal perikarya with high levels of CO reaction product in both deprived puff and interpuff areas. This effect was less marked in the deprived puffs of monkeys lid-sutured for 2.5 and 3 yrs, suggesting recovery of CO activity in some neurons. Neurons in deprived puffs and interpuffs were generally similar in size to those in nondeprived regions, although CO-reactive cells were significantly smaller in the deprived puffs of monkeys enucleated for 28.5 or 60 wks. These results indicate that the metabolic response of neuronal elements in supragranular striate cortex depends upon the nature of the visual deficit. The partial sparing of CO reactivity in deprived puffs of lid-sutured monkeys may reflect the continued transmission of certain types of visual stimuli through a closed eyelid.
在成年猕猴中，研究了在经历不同时长单眼摘除、眼睑缝合以及用河豚毒素（TTX）阻断视网膜冲动后的猕猴纹状皮层颗粒上层小体中细胞色素氧化酶（CO）反应性的层状结构和细胞分布。与眼睑缝合的猕猴的剥夺小体中较慢且不太明显的减少相比，摘除眼球和使用TTX导致由摘除或处理的眼睛主导的小体中CO反应区域的大小迅速且严重丧失。在所有剥夺动物中，剥夺小体的横截面积在上层（2层和3A层）减少最快。在长期摘除眼球（60周）和TTX处理（4周）的猕猴中，3B层的小体面积严重减少，而在眼睑缝合的猕猴中3B层的反应性似乎部分保留。在所有检查的猕猴中，整个剥夺小体中CO反应产物的密度显著且均匀降低；然而，在眼睑缝合11周的成年猕猴中，这种降低不太严重。虽然没有获得细胞丢失的证据，但所有三种形式的视觉剥夺都导致在剥夺小体和小体间区域中具有高水平CO反应产物的神经元胞体数量减少。这种效应在眼睑缝合2.5年和3年的猕猴的剥夺小体中不太明显，表明一些神经元的CO活性有所恢复。剥夺小体和小体间的神经元大小通常与非剥夺区域的神经元相似，尽管在摘除眼球28.5周或60周的猕猴的剥夺小体中，CO反应性细胞明显较小。这些结果表明，颗粒上层纹状皮层中神经元成分的代谢反应取决于视觉缺陷的性质。眼睑缝合的猕猴的剥夺小体中CO反应性的部分保留可能反映了某些类型的视觉刺激通过闭合眼睑的持续传递。
