McDonald J W, Trescher W H, Johnston M V
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD.
Brain Res. 1990 Aug 27;526(1):165-8. doi: 10.1016/0006-8993(90)90266-e.
Direct unilateral intrastriatal stereotaxic injections of the selective ionotropic-type quisqualate receptor agonist, AMPA, in postnatal day 7 rats produced tonic-clonic seizure activity. Quantitative analysis of the severity of brain injury was assessed by comparison of the disparities in the weights of injected and contralateral cerebral hemispheres 3 days after the excitotoxin injection. The amount of AMPA that produced half-maximal brain injury was 9.5 nmol as assessed by comparison of disparities in cerebral hemisphere weights. In contrast, quisqualate was 26 times less potent. The marked susceptibility of the developing rat brain to AMPA toxicity may provide a useful model to assess the neuroprotective effectiveness and selectivity of ionotropic quisqualate receptor antagonists.
在出生后第7天的大鼠中，通过立体定位法向单侧纹状体内直接注射选择性离子型quisqualate受体激动剂AMPA，可产生强直阵挛性癫痫发作活动。在注射兴奋性毒素3天后，通过比较注射侧和对侧大脑半球的重量差异，对脑损伤的严重程度进行定量分析。通过比较大脑半球重量差异评估，产生半数最大脑损伤的AMPA量为9.5纳摩尔。相比之下，quisqualate的效力要低26倍。发育中的大鼠大脑对AMPA毒性的显著易感性，可能为评估离子型quisqualate受体拮抗剂的神经保护有效性和选择性提供一个有用的模型。
